The primary focus of the Margolis laboratory is research on the cellular and molecular mechanisms that regulate the establishment and maintenance of latent neuronal infection with herpes simplex virus (HSV). Ongoing research is aimed at documenting the role of both neuronal and viral gene expression in the establishment and maintenance of HSV latency. The ultimate goal of this work is to gain enough of an understanding about the regulation of HSV latent infection that therapeutic interventions can be devised to eliminate latent infection or prevent viral reactivation. A second line of investigation focuses on development and evaluation of molecular diagnostics for infectious eye disease. A third line of investigation focuses on the use of telemedicine to screen AIDS patients for CMV retinitis in developing countries.