wustl.edu

Support for our endeavors
comes primarily from the
National Institutes of Health

Methods

Molecular Biology

  • Yeast one-hybrid assays for protein-DNA interaction
  • Yeast two-hybrid assays for protein-protein interaction
  • Site-directed mutagenesis
  • Recombinant protein expression and purification
  • Co-immunoprecipitation
  • Chromatin immunoprecipitation (ChIP) to study in vivo targets of transcription factors
  • In vitro protein-DNA binding assays: EMSA and footprinting

Functional Analysis

  • Transient cell transfection assays with luciferase reporters
  • Double-strand DNA interference (RNAi)
  • Transgenic and knockout mouse studies
  • Functional analysis of mutant proteins carrying genetically identified human mutations

Gene Expression Analysis

  • Quantitative and qualitative RT-PCR analysis
  • In situ hybridization on retinal sections
  • Microarray (chip)
  • Western blots
  • Immunohistochemistry
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The Vision Research Community at Washington University in St. Louis
Washington University School of Medicine
660 S.Euclid Ave., St. Louis, Missouri 63110
Campus Box 8096, McMillian Building, St. Louis, Missouri 63110